diff --git a/core/config/project_settings.cpp b/core/config/project_settings.cpp
index 9f3ffac5679..9cff93007ce 100644
--- a/core/config/project_settings.cpp
+++ b/core/config/project_settings.cpp
@@ -1506,6 +1506,12 @@ ProjectSettings::AutoloadInfo ProjectSettings::get_autoload(const StringName &p_
 	return autoloads[p_name];
 }
 
+void ProjectSettings::fix_autoload_paths() {
+	for (KeyValue<StringName, AutoloadInfo> &kv : autoloads) {
+		kv.value.path = ResourceUID::ensure_path(kv.value.path);
+	}
+}
+
 const HashMap<StringName, String> &ProjectSettings::get_global_groups_list() const {
 	return global_groups;
 }
diff --git a/core/config/project_settings.h b/core/config/project_settings.h
index a0962a61560..a861f49f461 100644
--- a/core/config/project_settings.h
+++ b/core/config/project_settings.h
@@ -223,6 +223,7 @@ public:
 	void remove_autoload(const StringName &p_autoload);
 	bool has_autoload(const StringName &p_autoload) const;
 	AutoloadInfo get_autoload(const StringName &p_name) const;
+	void fix_autoload_paths();
 
 	const HashMap<StringName, String> &get_global_groups_list() const;
 	void add_global_group(const StringName &p_name, const String &p_description);
diff --git a/main/main.cpp b/main/main.cpp
index 2c4983881ad..750b93cca8d 100644
--- a/main/main.cpp
+++ b/main/main.cpp
@@ -2201,7 +2201,8 @@ Error Main::setup(const char *execpath, int argc, char *argv[], bool p_second_ph
 	initialize_modules(MODULE_INITIALIZATION_LEVEL_CORE);
 	register_core_extensions(); // core extensions must be registered after globals setup and before display
 
-	ResourceUID::get_singleton()->load_from_cache(true); // load UUIDs from cache.
+	ResourceUID::get_singleton()->load_from_cache(true); // Load UUIDs from cache.
+	ProjectSettings::get_singleton()->fix_autoload_paths(); // Handles autoloads saved as UID.
 
 	if (ProjectSettings::get_singleton()->has_custom_feature("dedicated_server")) {
 		audio_driver = NULL_AUDIO_DRIVER;